Composite
Part:BBa_K327010:Design
Designed by: Holly Lam Group: iGEM10_UC_Davis (2010-09-28)
light sensor + RFP + lambda cI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1860
Illegal NheI site found at 1883 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1702
Illegal BglII site found at 4171
Illegal BamHI site found at 65
Illegal XhoI site found at 2278 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1255
Illegal AgeI site found at 5011
Illegal AgeI site found at 5123 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
light sensor differs from M30109; the second promoter has been replaced with a constitutive promoter to enable use of TetR elsewhere in the system.
Source
Registry