Composite

Part:BBa_K327010:Design

Designed by: Holly Lam   Group: iGEM10_UC_Davis   (2010-09-28)


light sensor + RFP + lambda cI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1860
    Illegal NheI site found at 1883
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1702
    Illegal BglII site found at 4171
    Illegal BamHI site found at 65
    Illegal XhoI site found at 2278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1255
    Illegal AgeI site found at 5011
    Illegal AgeI site found at 5123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

light sensor differs from M30109; the second promoter has been replaced with a constitutive promoter to enable use of TetR elsewhere in the system.


Source

Registry

References